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Data sheet of RPS6KA1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Dog,Horse,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about RPS6KA1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16907 |
| Product name: | RPS6KA1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA1 . |
| Gene id: | 6195 |
| Gene name: | RPS6KA1 |
| Gene alias: | HU-1|MAPKAPK1A|RSK|RSK1 |
| Gene description: | ribosomal protein S6 kinase, 90kDa, polypeptide 1 |
| Immunogen: | A synthetic peptide corresponding to a region near C-terminus end of human RPS6KA1 . |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Dog,Horse,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using RPS6KA1 polyclonal antibody (Cat # PAB16907) shows detection of a band ~90 KDa in size corresponding to RPS6KA1 (arrowhead) in EGF stimulated (Lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (Lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1 : 1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase.Chaturvedi D, Poppleton HM, Stringfield T, Barbier A, Patel TB. Mol Cell Biol. 2006 Jun;26(12):4586-600. |