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TRAF2 polyclonal antibody

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Data sheet of TRAF2 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse
Host speciesRabbit
ApplicationsELISA,WB-Ce,IF

More info about TRAF2 polyclonal antibody

Brand: Abnova
Reference: PAB16905
Product name: TRAF2 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of TRAF2 .
Gene id: 7186
Gene name: TRAF2
Gene alias: MGC:45012|TRAP|TRAP3
Gene description: TNF receptor-associated factor 2
Immunogen: A synthetic peptide corresponding to internal region of human TRAF2 .
Form: Liquid
Recommend dilutions: ELISA (1:60000-1:250000)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse
Application image: PAB16905-46-1-1.jpg
Application image note: Western blot using TRAF2 polyclonal antibody (Cat # PAB16905) shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 1 shows endogenous TRAF2 detected with antibody at 47 KDa (arrowhead). Lane 2 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 1 is unknown. Briefly, each lane contains approximately 14 ug of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1 : 500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1 : 20,000 dilution conjugated Gt-a-Rabbit DyLight 649 for 1 hr at room temperature. Fluorescence image was captured using the VersaDoc®Imaging System developed by Bio-Rad.
Applications: ELISA,WB-Ce,IF
Shipping condition: Dry Ice
Publications: RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling.Li S, Wang L, Berman MA, Zhang Y, Dorf ME.
Mol Cell. 2006 Nov 17;24(4):497-509.

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