Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human,Mouse |
Host species | Rabbit |
Applications | ELISA,WB-Ce,IF |
Brand: | Abnova |
Reference: | PAB16905 |
Product name: | TRAF2 polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of TRAF2 . |
Gene id: | 7186 |
Gene name: | TRAF2 |
Gene alias: | MGC:45012|TRAP|TRAP3 |
Gene description: | TNF receptor-associated factor 2 |
Immunogen: | A synthetic peptide corresponding to internal region of human TRAF2 . |
Form: | Liquid |
Recommend dilutions: | ELISA (1:60000-1:250000) Western Blot (1 ug/mL) The optimal working dilution should be determined by the end user. |
Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human,Mouse |
Application image: | ![]() |
Application image note: | Western blot using TRAF2 polyclonal antibody (Cat # PAB16905) shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 1 shows endogenous TRAF2 detected with antibody at 47 KDa (arrowhead). Lane 2 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 1 is unknown. Briefly, each lane contains approximately 14 ug of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1 : 500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1 : 20,000 dilution conjugated Gt-a-Rabbit DyLight 649 for 1 hr at room temperature. Fluorescence image was captured using the VersaDoc®Imaging System developed by Bio-Rad. |
Applications: | ELISA,WB-Ce,IF |
Shipping condition: | Dry Ice |
Publications: | RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling.Li S, Wang L, Berman MA, Zhang Y, Dorf ME. Mol Cell. 2006 Nov 17;24(4):497-509. |