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Data sheet of SAE1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about SAE1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16904 |
| Product name: | SAE1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SAE1. |
| Gene id: | 10055 |
| Gene name: | SAE1 |
| Gene alias: | AOS1|FLJ3091|HSPC140|SUA1 |
| Gene description: | SUMO1 activating enzyme subunit 1 |
| Immunogen: | A synthetic peptide corresponding to a region surrounding S185 of human SAE1. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using SAE1 polyclonal antibody (Cat # PAB16904) shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1 : 1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.Desterro JM, Rodriguez MS, Kemp GD, Hay RT. J Biol Chem. 1999 Apr 9;274(15):10618-24. |