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Data sheet of SMAD1 (phospho S206) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about SMAD1 (phospho S206) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16903 |
| Product name: | SMAD1 (phospho S206) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD1. |
| Gene id: | 4086 |
| Gene name: | SMAD1 |
| Gene alias: | BSP1|JV4-1|JV41|MADH1|MADR1 |
| Gene description: | SMAD family member 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding to S206 of human SMAD1. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human SMAD1 phosphoS206 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human SMAD1 is minimal by ELISA and western blot. |
| Reactivity: | Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using SMAD1 (phospho S206) polyclonal antibody (Cat # PAB16903) shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from human hepatoma (HepG2, lanes 1-4) derived cell lines treated with PBS, BMP2 (5 ng/mL), EGF (1 ng/mL), or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1 : 500 dilution in 1% BLOTTO and reacted for 1 hour at room temperature. Primary antibody was pre-incubated before reacting with blot as follows : top row - with PBS, middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. The membrane was washed and reacted with a 1 : 3,000 dilution HRP-conjugated a-Rabbit IgG for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Balancing BMP signaling through integrated inputs into the Smad1 linker.Sapkota G, Alarcon C, Spagnoli FM, Brivanlou AH, Massague J. Mol Cell. 2007 Feb 9;25(3):441-54. |