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Data sheet of GPC1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about GPC1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB16896 |
| Product name: | GPC1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of GPC1 . |
| Gene id: | 2817 |
| Gene name: | GPC1 |
| Gene alias: | FLJ38078|glypican |
| Gene description: | glypican 1 |
| Immunogen: | A synthetic peptide corresponding to internal region of human GPC1 . |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using GPC1 polyclonal antibody (Cat # PAB16896) shows detection of exogenous GPC1 in 50 ug 293T whole cell lysate (Lane 2). Minor bands may represent post translational modifications of GPC1. Lane 1 : untransfected 293T cell lysate. The blot was incubated overnight with primary antibody at a 1 : 1,000 dilution followed by washes and exposure to the secondary antibody HRP Gt-a-Rabbit IgG diluted 1 : 5,000 in 5% BLOTTO at 4°C.Personal Communication, Caterina Bianco, CCR-NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | A Nodal- and ALK4-independent signaling pathway activated by Cripto-1 through Glypican-1 and c-Src.Bianco C, Strizzi L, Rehman A, Normanno N, Wechselberger C, Sun Y, Khan N, Hirota M, Adkins H, Williams K, Margolis RU, Sanicola M, Salomon DS. Cancer Res. 2003 Mar 15;63(6):1192-7. |