Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human |
Host species | Rabbit |
Applications | Dot-Pep |
Brand: | Abnova |
Reference: | PAB1657 |
Product name: | BRAF (phospho T400) polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF. |
Gene id: | 673 |
Gene name: | BRAF |
Gene alias: | B-RAF1|BRAF1|FLJ95109|MGC126806|MGC138284|RAFB1 |
Gene description: | v-raf murine sarcoma viral oncogene homolog B1 |
Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T400 of human BRAF. |
Protein accession: | NP_004324;P15056 |
Form: | Liquid |
Recommend dilutions: | Dot Blot (1:500) The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS (0.09% sodium azide) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Reactivity: | Human |
Application image: | ![]() |
Application image note: | Dot blot analysis of BRAF (phospho T400) polyclonal antibody (Cat # PAB1657) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. |
Applications: | Dot-Pep |
Shipping condition: | Dry Ice |
Publications: | Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype.Yamaguchi T, Wallace DP, Magenheimer BS, Hempson SJ, Grantham JJ, Calvet JP. J Biol Chem. 2004 Sep 24;279(39):40419-30. Epub 2004 Jul 19. |