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Dnmt1 polyclonal antibody

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Data sheet of Dnmt1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityFrog,Human,Mouse
Host speciesRabbit
ApplicationsWB,IP,WB-Ce,IF

More info about Dnmt1 polyclonal antibody

Brand: Abnova
Reference: PAB15590
Product name: Dnmt1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against partial recombinant Dnmt1.
Gene id: 13433
Gene name: Dnmt1
Gene alias: Cxxc9|Dnmt|Dnmt1o|MTase|Met-1|Met1|MommeD2
Gene description: DNA methyltransferase (cytosine-5) 1
Immunogen: Recombinant protein corresponding to amino acids 1-248 mouse Dnmt1.
Form: Liquid
Recommend dilutions: Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In antiserum (0.05% sodium azide)
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Mouse
Specificity: This antibody reacts with Dnmt1.
Reactivity: Frog,Human,Mouse
Application image: PAB15590-46-1-1.jpg
Application image note: Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts.
Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene.
Biotylated Np95 was captured by streptoavidin beads.
The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 and Pcna.
( The data were kindly provided by Dr. M. Muto of Riken Research Center for Allergy and Immunology ).
Applications: WB,IP,WB-Ce,IF
Shipping condition: Dry Ice
Publications: Epigenetics of eu- and heterochromatin in inverted and conventional nuclei from mouse retina.Eberhart A, Feodorova Y, Song C, Wanner G, Kiseleva E, Furukawa T, Kimura H, Schotta G, Leonhardt H, Joffe B, Solovei I
Chromosome Res. 2013 Aug;21(5):535-54. doi: 10.1007/s10577-013-9375-7. Epub 2013 Aug 31.

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