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Data sheet of CASP9 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Dog,Gerbils,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,IP,IHC-Fr,IHC-P,IEM |
More info about CASP9 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB14669 |
| Product name: | CASP9 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against recombinant CASP9. |
| Gene id: | 842 |
| Gene name: | CASP9 |
| Gene alias: | APAF-3|APAF3|CASPASE-9c|ICE-LAP6|MCH6 |
| Gene description: | caspase 9, apoptosis-related cysteine peptidase |
| Immunogen: | Recombinant protein corresponding to human CASP9. |
| Protein accession: | NP_001220 |
| Form: | Liquid |
| Recommend dilutions: | Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:5000) Immunoprecipitation (1:50-1:200) Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In serum (0.05% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Caspase-9 immunostaining may appear diffuse or punctate. It may be localized in the cytosol, mitochondria, and nucleus. Caspase-9 staining in the nucleus is considered to be an indication of active Caspase-9. Both pro and active Caspase-9 staining may also be seen in the mitochondria. Caspase antibodies are classical tools for detecting inactive (pro) and active (cleaved) forms of the enzymes. The presence of the large or small subunits in western blots is considered to be a marker of caspase activation. |
| Reactivity: | Dog,Gerbils,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Formalin-fixed, paraffin-embedded human brain sections stained for Active/Cleaved CASP9 expression using CASP9 polyclonal antibody (Cat #PAB14669) at 1 : 2000. A and B. The pattern of CASP9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain. |
| Applications: | WB,IP,IHC-Fr,IHC-P,IEM |
| Shipping condition: | Dry Ice |
| Publications: | Identifying the practice characteristics of advanced practitioners in acute and critical care settings.Woods LP. Intensive Crit Care Nurs. 1998 Dec;14(6):308-17. |