Tph1 (phospho S260) polyclonal antibody View larger

Tph1 (phospho S260) polyclonal antibody

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Data sheet of Tph1 (phospho S260) polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Chicken,Dog,Human,Mouse,Rat,Zebra fish
Host speciesRabbit
ApplicationsWB-Ti

More info about Tph1 (phospho S260) polyclonal antibody

Brand: Abnova
Reference: PAB14527
Product name: Tph1 (phospho S260) polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of Tph1.
Gene id: 24848
Gene name: Tph1
Gene alias: Tph
Gene description: tryptophan hydroxylase 1
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S260 of rat Tph1.
Protein accession: P09810
Form: Liquid
Recommend dilutions: Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Rat
Specificity: Specific for the ~55 KDa tryptophan hydroxylase protein phosphorylated at Ser260. It is anticipated that This antibody will react with bovine, canine, chicken, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Reactivity: Bovine,Chicken,Dog,Human,Mouse,Rat,Zebra fish
Application image: PAB14527-47-L9-1.jpg
Application image note: Western blot of rat brainstem lysate showing specific immunolabeling of the ~55k Tph1 protein phosphorylated at Ser260. The labeling is specifically blocked by the phosphopeptide (Phos-pep, lane 1) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep, lane 2) did not block the immunolabeling.
Applications: WB-Ti
Shipping condition: Dry Ice
Publications: Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling.Jiang GC, Yohrling GJ 4th, Schmitt JD, Vrana KE.
J Mol Biol. 2000 Sep 29;302(4):1005-17.

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