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Data sheet of H3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP,Dot |
More info about H3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB14096 |
| Product name: | H3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of H3. |
| Immunogen: | A mixture of synthetic peptides (conjugated with KLH) that corresponding to internal region and to C-terminus of unmodified histone H3. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100-1:1000) Dot Blot (1:1000) Western Blot (1:250) ChIP (1-5 ul/ChIP) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In serum (0.05% sodium azide) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | A Dot Blot analysis was performed to test the cross reactivity of H3 polyclonal antibody (Cat # PAB14096) with the peptides used for immunization of the rabbit and other peptides containing different modifications or unmodified sequences of histone H3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1 : 1,000. Figure shows a high specificity of the crude serum for one of the unmodified peptides. |
| Applications: | WB,ELISA,ChIP,Dot |
| Shipping condition: | Dry Ice |
| Publications: | Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.Zheng C, Hayes JJ. J Biol Chem. 2003 Jun 27;278(26):24217-24. Epub 2003 Apr 15. |