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Data sheet of LCK (phospho Y505) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about LCK (phospho Y505) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12750 |
| Product name: | LCK (phospho Y505) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of LCK. |
| Gene id: | 3932 |
| Gene name: | LCK |
| Gene alias: | YT16|p56lck|pp58lck |
| Gene description: | lymphocyte-specific protein tyrosine kinase |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y505 of human, rat, bovine and dog LCK. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Bovine,Rat,Dog |
| Specificity: | This antibody recognizes LCK with a phosphorylated site of Tyrosine 505. It does not cross-react with non-phosphospecific peptide. |
| Reactivity: | Bovine,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The whole cell lysate derived from serum starved Jurkat was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by LCK (phospho Y505) polyclonal antibody (Cat # PAB12750) at 1 : 500. An immunoreactive band around ~ 60 kDa is observed. |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Suppression of proximal T cell receptor signaling and lytic function in CD8+ tumor-infiltrating T cells.Monu N, Frey AB. Cancer Res. 2007 Dec 1;67(23):11447-54. |