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Data sheet of KDR polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IHC |
More info about KDR polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12746 |
| Product name: | KDR polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of KDR. |
| Gene id: | 3791 |
| Gene name: | KDR |
| Gene alias: | CD309|FLK1|VEGFR|VEGFR2 |
| Gene description: | kinase insert domain receptor (a type III receptor tyrosine kinase) |
| Immunogen: | A synthetic peptide corresponding to residues surrounding Y951 (KDYVG) of human KDR. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~200 KDa of human KDR without a phosphorylation site of Tyrosine 951. It does not recognize phosphorylated peptides. |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | The whole cell lysate derived from human umbilical vein endothelial cell (HUVEC) was stimulated by KDR for 20 min, then immunoprecipitated by KDR polyclonal antibody (Cat # PAB12647) followed by immune-probing with KDR polyclonal antibody (Cat # PAB12746) at 1 : 500. An immunoreactive band is observed around ~200 KDa (Lane 1). The lane 2 is a negative control. |
| Applications: | WB,IP,ELISA,IHC |
| Shipping condition: | Dry Ice |
| Publications: | VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis.Matsumoto T, Bohman S, Dixelius J, Berge T, Dimberg A, Magnusson P, Wang L, Wikner C, Qi JH, Wernstedt C, Wu J, Bruheim S, Mugishima H, Mukhopadhyay D, Spurkland A, Claesson-Welsh L. EMBO J. 2005 Jul 6;24(13):2342-53. Epub 2005 Jun 16. |