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Data sheet of Trimethyl-Histone H3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IHC,IF |
More info about Trimethyl-Histone H3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12740 |
| Product name: | Trimethyl-Histone H3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Trimethyl-Histone H3. |
| Immunogen: | A synthetic peptide corresponding to trimethyl lysine 27 of human Histone H3. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody only recognizes ~ 17 KDa of Trimethylated Histone H3 at K27. It does not cross react to non-methylated H3 or mono-, di-methylated Histone H3. |
| Reactivity: | Bovine,Chicken,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740), then Cy3 labeled Gt anti Rb IgG for visualization under fluorescent microscope. |
| Applications: | WB,IP,ELISA,IHC,IF |
| Shipping condition: | Dry Ice |
| Publications: | Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL. Nat Struct Mol Biol. 2007 Mar;14(3):240-2. Epub 2007 Feb 18. |