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MTOR polyclonal antibody

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Data sheet of MTOR polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesRabbit
ApplicationsWB,IP,ELISA,IHC

More info about MTOR polyclonal antibody

Brand: Abnova
Reference: PAB12715
Product name: MTOR polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of MTOR.
Gene id: 2475
Gene name: MTOR
Gene alias: FRAP|FRAP1|FRAP2|RAFT1|RAPT1
Gene description: mechanistic target of rapamycin
Immunogen: A synthetic peptide corresponding to C-terminus of human MTOR.
Form: Liquid
Recommend dilutions: Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage instruction: Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This antibody only recognizes ~290 KDa of human MTOR.
Reactivity: Human,Mouse,Rat
Application image: PAB12715-14-7-1.jpg
Application image note: The cell lysate derived from MCF-7 was immuno-precipitated by MTOR polyclonal antibody (Cat # PAB12715), resolved onto 7.5% SDS-PAGE, transferred onto NC membrane, then immuno-blotted by MTOR polyclonal antibody (Cat # PAB12715) at 1 : 500.
An immunoreactive band around ~290 kDa was observed (lane 1). Lane 2 is a negative control.
Applications: WB,IP,ELISA,IHC
Shipping condition: Dry Ice
Publications: Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function.Oshiro N, Yoshino K, Hidayat S, Tokunaga C, Hara K, Eguchi S, Avruch J, Yonezawa K.
Genes Cells. 2004 Apr;9(4):359-66.

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