Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Human,Mouse,Rat |
Host species | Rabbit |
Applications | WB,IP,ELISA,ChIP,IHC,IF |
Brand: | Abnova |
Reference: | PAB12679 |
Product name: | HIST2H3C polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of HIST2H3C. |
Gene id: | 126961 |
Gene name: | HIST2H3C |
Gene alias: | H3|H3.2|H3/M|H3F2|H3FM|MGC9629 |
Gene description: | histone cluster 2, H3c |
Immunogen: | A synthetic peptide corresponding to dimethyl lysine 27 of human HIST2H3C. |
Form: | Liquid |
Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user. |
Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Human |
Specificity: | This antibody only recognizes ~17 KDa of Dimethylated HIST2H3C. It does not cross react to non-methylated H3 or mono-, Tri-methylated Histone H3. |
Reactivity: | Human,Mouse,Rat |
Application image: | ![]() |
Application image note: | Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL HIST2H3C polyclonal antibody (Cat # PAB12679), then FITC labeled Goat anti Rabbit IgG for visualization under fluorescent microscope. |
Applications: | WB,IP,ELISA,ChIP,IHC,IF |
Shipping condition: | Dry Ice |
Publications: | Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL. Nat Struct Mol Biol. 2007 Mar;14(3):240-2. Epub 2007 Feb 18. |