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HIST2H3C polyclonal antibody

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Data sheet of HIST2H3C polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesRabbit
ApplicationsWB,IP,ELISA,ChIP,IHC,IF

More info about HIST2H3C polyclonal antibody

Brand: Abnova
Reference: PAB12679
Product name: HIST2H3C polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of HIST2H3C.
Gene id: 126961
Gene name: HIST2H3C
Gene alias: H3|H3.2|H3/M|H3F2|H3FM|MGC9629
Gene description: histone cluster 2, H3c
Immunogen: A synthetic peptide corresponding to dimethyl lysine 27 of human HIST2H3C.
Form: Liquid
Recommend dilutions: Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
Storage buffer: In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage instruction: Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This antibody only recognizes ~17 KDa of Dimethylated HIST2H3C. It does not cross react to non-methylated H3 or mono-, Tri-methylated Histone H3.
Reactivity: Human,Mouse,Rat
Application image: PAB12679-49-8-1.jpg
Application image note: Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 ug/mL HIST2H3C polyclonal antibody (Cat # PAB12679), then FITC labeled Goat anti Rabbit IgG for visualization under fluorescent microscope.
Applications: WB,IP,ELISA,ChIP,IHC,IF
Shipping condition: Dry Ice
Publications: Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins.Seward DJ, Cubberley G, Kim S, Schonewald M, Zhang L, Tripet B, Bentley DL.
Nat Struct Mol Biol. 2007 Mar;14(3):240-2. Epub 2007 Feb 18.

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