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Data sheet of AKT1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about AKT1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12650 |
| Product name: | AKT1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of AKT1. |
| Gene id: | 207 |
| Gene name: | AKT1 |
| Gene alias: | AKT|MGC99656|PKB|PKB-ALPHA|PRKBA|RAC|RAC-ALPHA |
| Gene description: | v-akt murine thymoma viral oncogene homolog 1 |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human AKT1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (0.5-2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody only recognizes ~60 KDa of human AKT1, not AKT1 or AKT2. It also reacts with mouse and rat AKT3 protein. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The cell lysate derived from HeLa cell line was immuno-blotted by AKT1 polyclonal antibody (Cat # PAB12650) at 1 : 500 (lane 1), Lane 2 : BSA as a negative control. |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines.Nakatani K, Thompson DA, Barthel A, Sakaue H, Liu W, Weigel RJ, Roth RA. J Biol Chem. 1999 Jul 30;274(31):21528-32. |