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Data sheet of RPS6KB1 (phospho S434) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about RPS6KB1 (phospho S434) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12645 |
| Product name: | RPS6KB1 (phospho S434) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of RPS6KB1. |
| Gene id: | 6198 |
| Gene name: | RPS6KB1 |
| Gene alias: | PS6K|S6K|S6K1|STK14A|p70(S6K)-alpha|p70-S6K|p70-alpha |
| Gene description: | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S434 of human RPS6KB1. |
| Immunogen sequence/protein sequence: | IRSPR |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~70 KDa of human RPS6KB1. It does not recognize non-phosphorylated peptides. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The cell lysate derived from conditioned NIH/3T3 cells was immunoprobed by RPS6KB1 (phospho S434) polyclonal antibody (Cat # PAB12645) at 1 : 500. An immunoreactive band is observed around ~ 70kDa (Lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2). |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Signaling from Akt to FRAP/TOR targets both 4E-BP and S6K in Drosophila melanogaster.Miron M, Lasko P, Sonenberg N. Mol Cell Biol. 2003 Dec;23(24):9117-26. |