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Data sheet of ATG5 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | IHC,WB-Ce,IHC-P,IF |
More info about ATG5 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12482 |
| Product name: | ATG5 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ATG5. |
| Gene id: | 9474 |
| Gene name: | ATG5 |
| Gene alias: | APG5|APG5-LIKE|APG5L|ASP|hAPG5 |
| Gene description: | ATG5 autophagy related 5 homolog (S. cerevisiae) |
| Immunogen: | A synthetic peptide corresponding to amino acids 1-50 of human ATG5. |
| Protein accession: | Q9H1Y0 |
| Form: | Liquid |
| Recommend dilutions: | Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL) Western Blot (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (30% glycerol, 0.09% sodium azide) |
| Storage instruction: | Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275. |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Western blot analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) using ATG5 polyclonal antibody (Cat # PAB12482). Lane 2 : ATG5 KO ES cell lysate (negative control). Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center. |
| Applications: | IHC,WB-Ce,IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.Pyo JO, Nah J, Kim HJ, Lee HJ, Heo J, Lee H, Jung YK. Autophagy. 2008 Apr 1;4(3):315-21. Epub 2008 Jan 7. |