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swi6 polyclonal antibody

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Data sheet of swi6 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityS. pombe
Host speciesRabbit
ApplicationsIP,ELISA,WB-Re

More info about swi6 polyclonal antibody

Brand: Abnova
Reference: PAB11378
Product name: swi6 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of swi6.
Gene id: 2541633
Gene name: swi6
Gene alias: SPAC824.10c
Gene description: chromodomain protein Swi6
Immunogen: A synthetic peptide corresponding to amino acids 314-328 of Schizosaccharomyces pombe swi6.
Protein accession: P40381;NP_593449
Form: Liquid
Recommend dilutions: ELISA (1:5000-1:30000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: S. pombe
Reactivity: S. pombe
Application image: PAB11378-50-outsr-1.jpg
Application image note: Western blot analysis is shown using swi6 polyclonal antibody (Cat # PAB11378) to detect endogenous protein present in S.pombe lysate (arrowhead).
Comparison to a molecular weight marker (not shown) indicatesa band of ~43 KDa corresponding to S.pombe Swi6 protein.
Approx. ~35 ug of lysate was loaded per lane onto a 4-20% gradient gel for SDS-PAGE followed by transfer to 0.45 um nitrocellulose.
The blot was incubated with a 1:1,700 dilution of the antibody at room temperature for 2 hrs.
Applications: IP,ELISA,WB-Re
Shipping condition: Dry Ice
Publications: Switching gene swi6, involved in repression of silent mating-type loci in fission yeast, encodes a homologue of chromatin-associated proteins from Drosophila and mammals.Lorentz A, Ostermann K, Fleck O, Schmidt H.
Gene. 1994 May 27;143(1):139-43.

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