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Data sheet of HUB1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about HUB1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11361 |
| Product name: | HUB1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against recombinant HUB1. |
| Gene id: | 855767 |
| Gene name: | HUB1 |
| Gene alias: | - |
| Gene description: | Hub1p |
| Immunogen: | Recombinant protein corresponding to Saccharomyces cerevisiae HUB1. |
| Protein accession: | Q6Q546 |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:1000-1:5000) Western Blot (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Immunoblot of HUB1 fusion protein. HUB1 polyclonal antibody (Cat # PAB11361) generated by immunization with recombinant yeast HUB1 was tested by immunoblot against yeast lysates expressing the HUB1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of HUB1 polyclonal antibody (Cat # PAB11361) (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-HUB1 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche) . Data contributed by M. Malakhov, www.lifesensors.com, personal communication. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Ubiquitin and its kin: how close are the family ties?Jentsch S, Pyrowolakis G. Trends Cell Biol. 2000 Aug;10(8):335-42. |