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Data sheet of CHK1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about CHK1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11360 |
| Product name: | CHK1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of CHK1. |
| Gene id: | 852577 |
| Gene name: | CHK1 |
| Gene alias: | - |
| Gene description: | Chk1p |
| Immunogen: | A synthetic peptide corresponding to amino acids 312-327 of Saccharomyces cerevisiae CHK1. |
| Protein accession: | P38147;NP_009833 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Western blot using CHK1 polyclonal antibody (Cat # PAB11360) shows detection of bands corresponding to CHK1 in Saccharomyces cerevisiae lysates. Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 KDa. Lane 2 shows a predominant band at ~38 KDa. Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively). Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 minat room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms.Sanchez Y, Bachant J, Wang H, Hu F, Liu D, Tetzlaff M, Elledge SJ. Science. 1999 Nov 5;286(5442):1166-71. |