Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of ATG12 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about ATG12 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11358 |
| Product name: | ATG12 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against recombinant ATG12. |
| Gene id: | 852518 |
| Gene name: | ATG12 |
| Gene alias: | APG12 |
| Gene description: | Atg12p |
| Immunogen: | Recombinant protein corresponding to Saccharomyces cerevisiae ATG12. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:1000-1:5000) Western Blot (1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Immunoblot of APG12 fusion protein. ATG12 polyclonal antibody (Cat # PAB11358) generated by immunization with recombinant yeast ATG12 was tested by immunoblot against yeast lysates expressing the ATG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with ATG12 using a 1:2,000 dilution of ATG12 polyclonal antibody (Cat # PAB11358) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4°C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-ATG12 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche) . Data contributed by M. Malakhov, Lifesensors Inc., personal communication. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation.Suzuki K, Kirisako T, Kamada Y, Mizushima N, Noda T, Ohsumi Y. EMBO J. 2001 Nov 1;20(21):5971-81. |