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Data sheet of SMT3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about SMT3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11357 |
| Product name: | SMT3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against recombinant SMT3. |
| Gene id: | 852122 |
| Gene name: | SMT3 |
| Gene alias: | - |
| Gene description: | Smt3p |
| Immunogen: | Recombinant protein corresponding to Saccharomyces cerevisiae SMT3. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:1000-1:5000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Immunoblot of SMT3-GFP fusion proteins cleaved by insect cell protein extracts. SMT3 polyclonal antibody (Cat # PAB11357), generated by immunization with recombinant yeast SMT3, was tested by immunoblot against several constructs of SMT3-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SMT3 and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E.coli expressed and purified SMT3-GFP fusion proteins after incubation with lysed cells (50 ugtotal protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60°C and were then re-probed using the anti-SMT3 antibody at a 1:1000 dilution incubated overnight at 4°C in 5% non-fat drymilk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | SUMO, ubiquitin's mysterious cousin.Muller S, Hoege C, Pyrowolakis G, Jentsch S. Nat Rev Mol Cell Biol. 2001 Mar;2(3):202-10. |