Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of MER2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | WB,IP,ELISA |
More info about MER2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11356 |
| Product name: | MER2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MER2. |
| Gene id: | 853478 |
| Gene name: | REC107 |
| Gene alias: | MER2 |
| Gene description: | Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation |
| Immunogen: | A synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae MER2. |
| Protein accession: | P21651 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:1000-1:10000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Specificity: | Reactivity occurs against Saccharomyces cerevisiae Mer2 protein and reactivity is independent of phosphorylation at residue S30. |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Western blot using MER2 polyclonal antibody (Cat # PAB11356) shows detection of phosphorylated and unphosphorylated MER2 in wild type, phosphatase treated and mutant cells. Lane 1 contains MER2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated MER2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains MER2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated MER2 at the S30 position. The primary antibody was used at a 1:5,000 dilution. Personal Communication. MichaelLichten, NIH, CCR, Bethesda, MD. |
| Applications: | WB,IP,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2.Cool M, Malone RE. Mol Cell Biol. 1992 Mar;12(3):1248-56. |