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ALDOA polyclonal antibody

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Data sheet of ALDOA polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityRabbit
Host speciesGoat
ApplicationsELISA,WB-Ce

More info about ALDOA polyclonal antibody

Brand: Abnova
Reference: PAB11351
Product name: ALDOA polyclonal antibody
Product description: Goat polyclonal antibody raised against native ALDOA.
Gene id: 100009055
Gene name: ALDOA
Gene alias: -
Gene description: aldolase A
Immunogen: Native purified ALDOA from rabbit muscle.
Form: Lyophilized
Recommend dilutions: Sandwich ELISA (1:10000-1:40000)
Immunoprecipitation (5 ul)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Native Purified Protein.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Goat
Antigen species / target species: Rabbit
Reactivity: Rabbit
Application image: PAB11351-46-outsr-1.jpg
Application image note: IgG purified ALDOA polyclonal antibody (Cat # PAB11351) to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot.
A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE.
The gel was transferred to nitrocellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk.
Color was allowed to develop using Super Signal West Pico Chemiluminescent Substrate (PIERCE).
This antibody clearly detects a band at ~41 KDa consistent with human aldolase.
Applications: ELISA,WB-Ce
Shipping condition: Dry Ice

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