S100A1/S100B polyclonal antibody View larger

S100A1/S100B polyclonal antibody

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Data sheet of S100A1/S100B polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Dog,Mouse,Rat
Host speciesRabbit
ApplicationsWB,ELISA,IHC-P

More info about S100A1/S100B polyclonal antibody

Brand: Abnova
Reference: PAB11341
Product name: S100A1/S100B polyclonal antibody
Product description: Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
Gene id: 528735|525716
Gene name: S100A1
Gene alias: MGC159706
Gene description: S100 calcium binding protein A1
Immunogen: Recombinant protein corresponding to full length bovine S100A1/S100B.
Protein accession: P02639 (Bovine gene ID : 528735)
Form: Lyophilized
Recommend dilutions: ELISA (1:5000-1:25000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:200-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Recombinant Protein.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Bovine
Specificity: A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
Reactivity: Bovine,Dog,Mouse,Rat
Application image: PAB11341-48-69-1.jpg
Application image note: Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B.
Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum.
Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.
Applications: WB,ELISA,IHC-P
Shipping condition: Dry Ice
Publications: Ions binding to S100 proteins: structural changes induced by calcium and zinc on S100a and S100b proteins.Baudier J, Gerard D.
Biochemistry. 1983 Jul 5;22(14):3360-9.

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