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Data sheet of Stat1 (phospho Y701) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about Stat1 (phospho Y701) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11306 |
| Product name: | Stat1 (phospho Y701) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Stat1. |
| Gene id: | 20846 |
| Gene name: | Stat1 |
| Gene alias: | 2010005J02Rik|AA408197 |
| Gene description: | signal transducer and activator of transcription 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y701 of mouse Stat1. |
| Protein accession: | NP_033309;P42225 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:25000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This antibody is specific to mouse Stat1 protein phosphorylated at Y701. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using Stat1 (phospho Y701) polyclonal antibody (Cat # PAB11306) shows detection of phos-phorylated Stat1 (indicated by arrowhead at ~91 kDa) in K-562 cells after 30 min treatment with 1 ku of hIFN-alpha (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The primary antibody at a 1:1,000 dilution at 4°C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Distinct modes of action applied by transcription factors STAT1 and IRF1 to initiate transcription of the IFN-gamma-inducible gbp2 gene.Ramsauer K, Farlik M, Zupkovitz G, Seiser C, Kroger A, Hauser H, Decker T. Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2849-54. Epub 2007 Feb 9. |