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Data sheet of SMAD3 (phospho S423/425) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Clawed frog,Frog,Human,Mouse,Pig,Rat,Zebra fish |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about SMAD3 (phospho S423/425) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11304 |
| Product name: | SMAD3 (phospho S423/425) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3. |
| Gene id: | 4088 |
| Gene name: | SMAD3 |
| Gene alias: | DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396 |
| Gene description: | SMAD family member 3 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S423/S425 of human SMAD3. |
| Protein accession: | NP_005893;P84022 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:75000-1:300000) Western Blot (1:2000-1:20000) Immunohistochemistry (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs against human Smad3 pS423 and pS425 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. |
| Reactivity: | Bovine,Chicken,Clawed frog,Frog,Human,Mouse,Pig,Rat,Zebra fish |
| Application image: |  |
| Application image note: | Immunohistochemical staining of SMAD3 (phospho S423/425) polyclonal antibody (Cat # PAB11304) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | CB2R orchestrates fibrogenesis through regulation of inflammatory response during the repair of skeletal muscle contusion.Zhang M, Jiang SK, Tian ZL, Wang M, Zhao R, Wang LL, Li SS, Liu M, Li JY, Zhang MZ, Guan DW. Int J Clin Exp Pathol 2015; 8(4):3491-3502 |