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Data sheet of ROCK2 (phospho Y256) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Tr |
More info about ROCK2 (phospho Y256) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11303 |
| Product name: | ROCK2 (phospho Y256) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of ROCK2. |
| Gene id: | 9475 |
| Gene name: | ROCK2 |
| Gene alias: | KIAA0619 |
| Gene description: | Rho-associated, coiled-coil containing protein kinase 2 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y256 of human ROCK2. |
| Protein accession: | BAA75636; O75116 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide). |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to human ROCK-2 protein phosphorylated at Y256. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using ROCK2 (phospho Y256) polyclonal antibody (Cat # PAB11303) shows detection of phosphorylated ROCK2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK2. The primary antibody was used at a 1 : 1000 dilution. Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD. |
| Applications: | ELISA,IHC,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.Takahashi N, Tuiki H, Saya H, Kaibuchi K. Genomics. 1999 Jan 15;55(2):235-7. |