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CCNT2 polyclonal antibody

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Data sheet of CCNT2 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Dog,Human,Mouse,Rat
Host speciesRabbit
ApplicationsIP,ELISA,WB-Ce

More info about CCNT2 polyclonal antibody

Brand: Abnova
Reference: PAB11292
Product name: CCNT2 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against partial recombinant CCNT2.
Gene id: 905
Gene name: CCNT2
Gene alias: FLJ90560|MGC134840
Gene description: cyclin T2
Immunogen: Recombinant protein corresponding to amino acids 1-584 of human CCNT2.
Protein accession: NP_001232(isoforma);NP_490595(isoformb)
Form: Liquid
Recommend dilutions: ELISA (1:5000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: In antiserum (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Recombinant Protein.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Bovine,Dog,Human,Mouse,Rat
Application image: PAB11292-46-1-1.jpg
Application image note: Western blot using CCNT2 polyclonal antibody (Cat # PAB11292) is shown to detect two major bands (arrowheads) corresponding to human CCN T2a and T2b as indicated.
Approximately 33 ug of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed forovernight at 4°C with the primary antibody diluted to 1 : 500 in 5% BLOTTO in PBS.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDyeâ„¢800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
IRDye™ 800 fluorescenceimage was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is atrademark of LI-COR, Inc.
Applications: IP,ELISA,WB-Ce
Shipping condition: Dry Ice
Publications: 7SK small nuclear RNA binds to and inhibits the activity of CDK9/cyclin T complexes.Nguyen VT, Kiss T, Michels AA, Bensaude O.
Nature. 2001 Nov 15;414(6861):322-5.

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