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Data sheet of WNT1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Dog,Human,Macaque,Mouse,Opossum,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about WNT1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11291 |
| Product name: | WNT1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of WNT1. |
| Gene id: | 7471 |
| Gene name: | WNT1 |
| Gene alias: | INT1 |
| Gene description: | wingless-type MMTV integration site family, member 1 |
| Immunogen: | A synthetic peptide corresponding to internal region of human WNT1. |
| Protein accession: | NP_005421.1;P04628 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:25000) Western Blot (1:1500-1:6000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Chicken,Dog,Human,Macaque,Mouse,Opossum,Rat |
| Application image: |  |
| Application image note: | Western blot using WNT1 polyclonal antibody (Cat # PAB11291) shows detection of endogenous WNT1 in human-derived MCF-7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to WNT1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1 : 1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with WNT1 (data not shown). |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | A Wnt-Axin2-GSK3beta cascade regulates Snail1 activity in breast cancer cells.Yook JI, Li XY, Ota I, Hu C, Kim HS, Kim NH, Cha SY, Ryu JK, Choi YJ, Kim J, Fearon ER, Weiss SJ. Nat Cell Biol. 2006 Dec;8(12):1398-406. Epub 2006 Oct 29. |