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UBA1 polyclonal antibody

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Data sheet of UBA1 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityDog,Human,Mouse,Rabbit,Rat
Host speciesRabbit
ApplicationsELISA,WB-Ce,IHC-P

More info about UBA1 polyclonal antibody

Brand: Abnova
Reference: PAB11287
Product name: UBA1 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against full length recombinant UBA1.
Gene id: 7317
Gene name: UBA1
Gene alias: A1S9|A1S9T|A1ST|AMCX1|GXP1|MGC4781|SMAX2|UBA1A|UBE1|UBE1X
Gene description: ubiquitin-like modifier activating enzyme 1
Immunogen: Recombinant GST fusion protein corresponding to full length human UBA1.
Protein accession: P22314;NP_003325
Form: Liquid
Recommend dilutions: ELISA (1:2000-1:10000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (2-20 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Recombinant Protein.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Dog,Human,Mouse,Rabbit,Rat
Application image: PAB11287-48-1-1.jpg
Application image note: Immunohistochemistry of UBA1 polyclonal antibody (Cat # PAB11287) was used at a 10 ug/mL to detect UBA1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (isletand exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis,thymus, tonsil, and uterus (glandular, stroma).
In many cells a punctate nuclear staining was observed.
Other cells showedboth cytoplasmic and nuclear staining.
This image shows UBA1 staining of human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communi-cation, TinaRoush, Life Span Biosciences, Seattle, WA.
Applications: ELISA,WB-Ce,IHC-P
Shipping condition: Dry Ice
Publications: A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter.Bres V, Kiernan RE, Linares LK, Chable-Bessia C, Plechakova O, Treand C, Emiliani S, Peloponese JM, Jeang KT, Coux O, Scheffner M, Benkirane M.
Nat Cell Biol. 2003 Aug;5(8):754-61.

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