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Data sheet of UBE2N polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Clawed frog,Dog,Frog,Human,Macaque,Mouse,Rat |
| Host species | Goat |
| Applications | ELISA,WB-Ti |
More info about UBE2N polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11286 |
| Product name: | UBE2N polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of UBE2N. |
| Gene id: | 7334 |
| Gene name: | UBE2N |
| Gene alias: | MGC131857|MGC8489|UBC13|UbcH-ben |
| Gene description: | ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast) |
| Immunogen: | A synthetic peptide corresponding to amino acids 40-51 of human UBE2N. |
| Protein accession: | NP_003339;P61088 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Chicken,Chimpanzee,Clawed frog,Dog,Frog,Human,Macaque,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using UBE2N polyclonal antibody (Cat # PAB11286) shows detection of UBE2N protein inhuman small intestine lysate (Lane 1), but not in mouse thymus lysate (Lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown, but could represent E2 complexes. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1 : 500 dilution. The membrane was washed and reacted with a 1 : 10,000 dilution of Alexa Fluorâ„¢ 680 conjugated Rb-a-Goat IgG. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex.Pastushok L, Moraes TF, Ellison MJ, Xiao W. J Biol Chem. 2005 May 6;280(18):17891-900. Epub 2005 Mar 4. |