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THRB polyclonal antibody

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Data sheet of THRB polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesRabbit
ApplicationsIP,ELISA,WB-Tr

More info about THRB polyclonal antibody

Brand: Abnova
Reference: PAB11277
Product name: THRB polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of THRB.
Gene id: 7068
Gene name: THRB
Gene alias: ERBA-BETA|ERBA2|GRTH|MGC126109|MGC126110|NR1A2|PRTH|THR1|THRB1|THRB2
Gene description: thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
Immunogen: A synthetic peptide corresponding to N-terminus of human THRB.
Protein accession: NP_000452.2;P10828
Form: Liquid
Recommend dilutions: ELISA (1:200000-1:220000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse,Rat
Application image: PAB11277-51-multi-1.jpg
Application image note: Western blot using THRB polyclonal antibody (Cat # PAB11277) shows detection of in vitro translated THRB (lane 2) and human thyroid stable cell line expressing THTRB (lane 3).
No staining is evident in non transfected human thyroid cells (lane 1), over expressed THRA (lane 4) or HeLa stable cell line expressing THRA (lane 5).
The band at ~62 kDa, indicated by the arrowheads, corresponds to THRB.
Personal communication, H. Ying, NCI, Bethesda, MD.
Applications: IP,ELISA,WB-Tr
Shipping condition: Dry Ice
Publications: Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture.Somogyi V, Horvath TL, Toth I, Bartha T, Frenyo LV, Kiss DS, Jocsak G, Kerti A, Naftolin F, Zsarnovszky A.
Acta Vet Hung. 2016 Dec 20;64(4):497-513.

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