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THRA polyclonal antibody

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Data sheet of THRA polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesRabbit
ApplicationsWB,IP,ELISA

More info about THRA polyclonal antibody

Brand: Abnova
Reference: PAB11276
Product name: THRA polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of THRA.
Gene id: 7067
Gene name: THRA
Gene alias: AR7|EAR7|ERB-T-1|ERBA|ERBA1|MGC000261|MGC43240|NR1A1|THRA1|THRA2|c-ERBA-1
Gene description: thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
Immunogen: A synthetic peptide corresponding to N-terminus of human THRA.
Protein accession: NP_955366;P10827-2
Form: Liquid
Recommend dilutions: ELISA (1:650000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Human,Mouse,Rat
Application image: PAB11276-12-multi-1.jpg
Application image note: Western blot using THRA polyclonal antibody (Cat # PAB11276) shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3).
No staining is evident in lysates from mock-transfected 293 cells (lane 2).
Endogenous THRA is not detected in mouse brain tissue lysate (lane 4).
Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus.
The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.
Applications: WB,IP,ELISA
Shipping condition: Dry Ice
Publications: Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture.Somogyi V, Horvath TL, Toth I, Bartha T, Frenyo LV, Kiss DS, Jocsak G, Kerti A, Naftolin F, Zsarnovszky A.
Acta Vet Hung. 2016 Dec 20;64(4):497-513.

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