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Data sheet of SPANXC polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Gorilla,Human |
| Host species | Rabbit |
| Applications | WB,ELISA |
More info about SPANXC polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11267 |
| Product name: | SPANXC polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SPANXC. |
| Gene id: | 64663 |
| Gene name: | SPANXC |
| Gene alias: | C|CTp11|SPANX-C |
| Gene description: | SPANX family, member C |
| Immunogen: | A synthetic peptide corresponding to amino acids 31-47 of human SPANXC. |
| Protein accession: | CAD29839;CAB75344;CAI41662;Q9NY87 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:50000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity-purified antibody is directed against human SPANX proteins. The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E. |
| Reactivity: | Chimpanzee,Gorilla,Human |
| Application image: |  |
| Application image note: | Western blot using SPANXC polyclonal antibody (Cat # PAB11267) shows detection of a band at ~17 KDacorresponding to SPANXC present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells arederived from a human melanoma and arepositive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANXC fusion protein. The right panel shows similar reactivity with purified recombinant SPANXB, SPANXC and SPANXN proteins. Proteins were separatedby SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1 : 1,000. IRDyeâ„¢800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers asindicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | The human SPANX multigene family: genomic organization, alignment and expression in male germ cells and tumor cell lines.Zendman AJ, Zschocke J, van Kraats AA, de Wit NJ, Kurpisz M, Weidle UH, Ruiter DJ, Weiss EH, van Muijen GN. Gene. 2003 May 8;309(2):125-33. |