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Data sheet of SMAD4 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Frog,Human,Mouse |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about SMAD4 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11265 |
| Product name: | SMAD4 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SMAD4. |
| Gene id: | 4089 |
| Gene name: | SMAD4 |
| Gene alias: | DPC4|JIP|MADH4 |
| Gene description: | SMAD family member 4 |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human SMAD4. |
| Protein accession: | BAB40977;Q13485 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:60000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Frog,Human,Mouse |
| Application image: |  |
| Application image note: | Western blot using SMAD4 polyclonal antibody (Cat # PAB11265) to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The primary antibody at a 1:1,000 dilution. Personal Communication, Kathleen Flanders, CCR-NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Smad4 is critical for self-renewal of hematopoietic stem cells.Karlsson G, Blank U, Moody JL, Ehinger M, Singbrant S, Deng CX, Karlsson S. J Exp Med. 2007 Mar 19;204(3):467-74. Epub 2007 Mar 12. |