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Data sheet of SMAD3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Tr |
More info about SMAD3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11264 |
| Product name: | SMAD3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SMAD3. |
| Gene id: | 4088 |
| Gene name: | SMAD3 |
| Gene alias: | DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396 |
| Gene description: | SMAD family member 3 |
| Immunogen: | A synthetic peptide corresponding to internal region of human SMAD3. |
| Protein accession: | NP_005893;P84022 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:50000) Western Blot (1:500-1:10000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is Smad3 specific, and reactivity to other Smad proteins (specifically Smad1, Smad2, Smad4, and Smad7) is not detected in over-expressed cell lysates. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using SMAD3 polyclonal antibody (Cat # PAB11264) to detect over-expressed SMAD3 in MDA-MB-231 cells (lane 2). Lane 1 shows mock infection of MDA-MB-231 cells with lentiviral vector alone. The primary antibody at a 1:5,000 dilution. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. |
| Applications: | ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | TGF-beta receptor-mediated signalling through Smad2, Smad3 and Smad4.Nakao A, Imamura T, Souchelnytskyi S, Kawabata M, Ishisaki A, Oeda E, Tamaki K, Hanai J, Heldin CH, Miyazono K, ten Dijke P. EMBO J. 1997 Sep 1;16(17):5353-62. |