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Data sheet of SMAD2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA |
More info about SMAD2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11262 |
| Product name: | SMAD2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SMAD2. |
| Gene id: | 4087 |
| Gene name: | SMAD2 |
| Gene alias: | JV18|JV18-1|MADH2|MADR2|MGC22139|MGC34440|hMAD-2|hSMAD2 |
| Gene description: | SMAD family member 2 |
| Immunogen: | A synthetic peptide corresponding to internal region of human SMAD2. |
| Protein accession: | NP_005892;Q15796 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100000) Western Blot (1:1000-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is Smad2 specific, and reactivity to other Smad proteins (specifically Smad1, Smad3, Smad4, and Smad7) is not detected in over-expressed cell lysates. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using SMAD2 polyclonal antibody (Cat # PAB11262) to detect over-expressed SMAD2 in COS cells (arrow). Lane 1 shows mock infection of COS cells with lentiviral vector alone. Lane 2 shows detection of SMAD2 in lysates of COS transfected with SMAD2. Lane 3 contains lysates of MDA-MB231 cells treated with vehicle. Lane 4 contains lysate of MDA-MB231 cells treated with TGF beta. The expected MW for SMAD2 is 52 kDa. The membrane was probed with the primary antibody at a 1:2500 dilution. Personal Communication Kathleen Flanders, CCR-NCI, Bethesda, MD. |
| Applications: | WB,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | Effect of the different phosphorylated Smad2 protein localizations on the invasive breast carcinoma phenotype.Liapis G, Mylona E, Alexandrou P, Giannopoulou I, Nikolaou I, Markaki S, Keramopoulos A, Nakopoulou L. APMIS. 2007 Feb;115(2):104-14. |