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Data sheet of SLIT3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ti |
More info about SLIT3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11260 |
| Product name: | SLIT3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SLIT3. |
| Gene id: | 6586 |
| Gene name: | SLIT3 |
| Gene alias: | FLJ10764|MEGF5|SLIL2|SLIT1|Slit-3|slit2 |
| Gene description: | slit homolog 3 (Drosophila) |
| Immunogen: | A synthetic peptide corresponding to amino acids 1164-1177 of human SLIT3. |
| Protein accession: | O75094;NP_003053 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Chicken,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using SLIT3 polyclonal antibody (Cat # PAB11260) shows detection ofa predominant band at ~145 KDa corresponding to SLIT3 (arrowhead) in a bovine thyroid tissue lysate using the 800 nm channel (green). Approximately 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™ 800 conjugated Gt-a-RabbitIgG [H&L] MXHu for 45 min atroom temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye isa trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening.Nakayama M, Nakajima D, Nagase T, Nomura N, Seki N, Ohara O. Genomics. 1998 Jul 1;51(1):27-34. |