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RNF34 polyclonal antibody

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Data sheet of RNF34 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityBovine,Dog,Human,Mouse,Orangutan,Rat
Host speciesRabbit
ApplicationsELISA,IF,WB-Tr

More info about RNF34 polyclonal antibody

Brand: Abnova
Reference: PAB11257
Product name: RNF34 polyclonal antibody
Product description: Rabbit polyclonal antibody raised against partial recombinant RNF34.
Gene id: 80196
Gene name: RNF34
Gene alias: FLJ21786|RFI|RIF|RIFF
Gene description: ring finger protein 34
Immunogen: Recombinant protein corresponding to amino acids 1-373 of human RNF34.
Protein accession: NP_919247;Q969K3
Form: Lyophilized
Recommend dilutions: ELISA (1:4000-1:20000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.
Storage buffer: Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Recombinant Protein.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Specificity: This protein A purified antibody is directed against human RNF34 protein. Expect reactivity with isoform 1 and 2 of RNF34.
Reactivity: Bovine,Dog,Human,Mouse,Orangutan,Rat
Application image: PAB11257-51-89-1.jpg
Application image note: Western blot using RNF34 polyclonal antibody (Cat # PAB11257) shows detection of human RNF34 (arrowhead) in lysate.
Lanes corresponding to empty vector 293T cell lysate (mock, lane 1) ; RNF34 transfected lysate (Lane 2) and RFFL transfected lysate (Lane 3), are shown using 20 ug of lysate per lane.
Lysates were prepared from equivalent numbers of cells.
The primary antibody diluted to 1:1,000.
Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
Applications: ELISA,IF,WB-Tr
Shipping condition: Dry Ice
Publications: Isolation and characterization of a novel gene, hRFI, preferentially expressed in esophageal cancer.Sasaki S, Nakamura T, Arakawa H, Mori M, Watanabe T, Nagawa H, Croce CM.
Oncogene. 2002 Jul 25;21(32):5024-30.

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