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Data sheet of MYL9 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ti |
More info about MYL9 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11255 |
| Product name: | MYL9 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MYL9. |
| Gene id: | 10398 |
| Gene name: | MYL9 |
| Gene alias: | LC20|MGC3505|MLC2|MRLC1|MYRL2 |
| Gene description: | myosin, light chain 9, regulatory |
| Immunogen: | A synthetic peptide corresponding to amino acids 12-27 of human MYL9. |
| Protein accession: | P24844(Human);Q9CQ19(Mouse) |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against the regulatory light chain of smooth and non- muscle myosin. This antibody detects both unphosphorylated and monophosphorylated forms of the protein. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using MYL9 polyclonal antibody (Cat # PAB11255) to detect vascular myosin (rat aorta, lane 1), but not cardiac myosin (mouse heart, lane2). Each lanewas loaded with 35 ug of lysate. Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to1 : 600 for 2 h at room temperature. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye 800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™ 800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | IP,ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Characterization and differential expression of human vascular smooth muscle myosin light chain 2 isoform in nonmuscle cells.Kumar CC, Mohan SR, Zavodny PJ, Narula SK, Leibowitz PJ. Biochemistry. 1989 May 2;28(9):4027-35. |