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Data sheet of POLK polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chimpanzee,Human,Mouse,Orangutan |
| Host species | Goat |
| Applications | ELISA,WB-Ce |
More info about POLK polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11246 |
| Product name: | POLK polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of POLK. |
| Gene id: | 51426 |
| Gene name: | POLK |
| Gene alias: | DINB1|DINP|POLQ |
| Gene description: | polymerase (DNA directed) kappa |
| Immunogen: | A synthetic peptide corresponding to amino acids 817-830 of human POLK. |
| Protein accession: | Q9UBT6 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:2000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | Expect reactivity with multiple splice variants of this protein. |
| Reactivity: | Chimpanzee,Human,Mouse,Orangutan |
| Application image: |  |
| Application image note: | Western blot using POLK polyclonal antibody (Cat # PAB11246) shows detection ofa band ~98 KDa corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed withthe primary antibody diluted to 1 : 1,000. Reaction occurred overnight at 4°C followed bywashes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at roomtemperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDyeis a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair.Haracska L, Prakash L, Prakash S. Genes Dev. 2003 Nov 15;17(22):2777-85. |