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PNKP polyclonal antibody

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Data sheet of PNKP polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityChimpanzee,Dog,Human,Mouse,Rat
Host speciesRabbit
ApplicationsWB,ELISA

More info about PNKP polyclonal antibody

Brand: Abnova
Reference: PAB11245
Product name: PNKP polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of PNKP.
Gene id: 11284
Gene name: PNKP
Gene alias: PNK
Gene description: polynucleotide kinase 3'-phosphatase
Immunogen: A synthetic peptide corresponding to internal region of human PNKP.
Protein accession: Q96T60;NP_009185
Form: Liquid
Recommend dilutions: ELISA (1:3000-1:12000)
Western Blot (1:300-1:1500)
The optimal working dilution should be determined by the end user.
Storage buffer: In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage instruction: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Quality control testing: Antibody Reactive Against Synthetic Peptide.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Human
Reactivity: Chimpanzee,Dog,Human,Mouse,Rat
Application image: PAB11245-12-multi-1.jpg
Application image note: Western blot using PNKP polyclonal antibody (Cat # PAB11245) shows detection of a 57 kDa band corresponding to :
Lane 1, human PNKP in a Y190 yeast cell lysate.
Lane 2, Y190 yeast cell lysate + human PNKP (Gal DNA BP).
Lane 3, EM9 XH Chinese hamster ovary cell lysate.
Lane 4, EM9 XH Chinese hamster ovary cell lysate + human PNK.
Lane 5, a HeLa cell lysate.
Approximately 10 ug of lysate was run on SDS-PAGE and reaction with a 1:1000 dilution of PNKP polyclonal antibody.
Applications: WB,ELISA
Shipping condition: Dry Ice
Publications: Molecular characterization of a human DNA kinase.Karimi-Busheri F, Daly G, Robins P, Canas B, Pappin DJ, Sgouros J, Miller GG, Fakhrai H, Davis EM, Le Beau MM, Weinfeld M.
J Biol Chem. 1999 Aug 20;274(34):24187-94.

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