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Data sheet of RELA (phospho S529) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about RELA (phospho S529) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10337 |
| Product name: | RELA (phospho S529) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA. |
| Gene id: | 5970 |
| Gene name: | RELA |
| Gene alias: | MGC131774|NFKB3|p65 |
| Gene description: | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S529 of human RELA. |
| Immunogen sequence/protein sequence: | PNGLLpSGDEDFC |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:10000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.1% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This phospho specific polyclonal antibody is specific to phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | TNF Induces phosphorylation of RELA in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using RELA (phospho S529) polyclonal antibody (Cat # PAB10337). Anti-beta actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Data contributed by Aggarwal BB, personal communication. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.Baldwin AS. J Clin Invest. 2001 Feb;107(3):241-6. |