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Data sheet of RELA (phospho S276) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about RELA (phospho S276) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10336 |
| Product name: | RELA (phospho S276) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA. |
| Gene id: | 5970 |
| Gene name: | RELA |
| Gene alias: | MGC131774|NFKB3|p65 |
| Gene description: | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S276 of human RELA. |
| Immunogen sequence/protein sequence: | QLRRPpSDRELSC |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:30000) Western Blot (1:1000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This phospho specific polyclonal antibody is specific to phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | TNF Induces phosphorylation of RELA in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using Phospho-RELA S276 polyclonal antibody (Cat # PAB10336). RELA polyclonal antibody (Cat # PAB10288) was used a control to show the presence of total RELA in both the cytoplasmic and nuclear extracts. Phosphorylation of RELA occurs after approximately 10 min of TNF exposure. Migration of phosphorylated RELA into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Personal Communication, Aggarwal BB. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Biological properties and growth in SCID mice of a new myelogenous leukemia cell line (KBM-5) derived from chronic myelogenous leukemia cells in the blastic phase.Beran M, Pisa P, O'Brien S, Kurzrock R, Siciliano M, Cork A, Andersson BS, Kohli V, Kantarjian H. Cancer Res. 1993 Aug 1;53(15):3603-10. |