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Data sheet of MLF1IP (phospho T78) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,IHC-P,WB-Tr |
More info about MLF1IP (phospho T78) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10333 |
| Product name: | MLF1IP (phospho T78) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of MLF1IP. |
| Gene id: | 79682 |
| Gene name: | MLF1IP |
| Gene alias: | CENP-50|CENP-U|CENP-U(50)|CENP50|CENPU|FLJ23468|KLIP1|PBIP1 |
| Gene description: | MLF1 interacting protein |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T78 of human MLF1IP. |
| Protein accession: | NP_078905;Q71F23 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:25000) Western Blot (1:500-1:2000) Immunocytochemistry (1:100-1:500) Immunohistochemistry (20 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to human MLF1IP protein phosphorylated at Thr78. |
| Reactivity: | Bovine,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333), stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006). |
| Applications: | ELISA,IHC-P,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation.Kang YH, Park JE, Yu LR, Soung NK, Yun SM, Bang JK, Seong YS, Yu H, Garfield S, Veenstra TD, Lee KS. Mol Cell. 2006 Nov 3;24(3):409-22. |