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Data sheet of CDC16 (phospho T580) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IF |
More info about CDC16 (phospho T580) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10325 |
| Product name: | CDC16 (phospho T580) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC16. |
| Gene id: | 8881 |
| Gene name: | CDC16 |
| Gene alias: | APC6 |
| Gene description: | cell division cycle 16 homolog (S. cerevisiae) |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T580 of human CDC16. |
| Protein accession: | Q13042 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human APC6 protein at the pT580 residue. This antibody is expected to react with all isoforms of this protein. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using CDC16 (phospho T580) polyclonal antibody (Cat # PAB10325) shows detectionof a band ~ 72 KDa corresponding to phosphorylated human CDC16 (arrowhead lane 1). Lane 1 -nocodazole treated HeLa whole cell lysate. Lane 2 -Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35 ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. |
| Applications: | ELISA,WB-Ce,IF |
| Shipping condition: | Dry Ice |
| Publications: | CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition.Tugendreich S, Tomkiel J, Earnshaw W, Hieter P. Cell. 1995 Apr 21;81(2):261-8. |