Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of PRKDC (phospho T2609) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Chimpanzee,Dog,Horse,Human,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce |
More info about PRKDC (phospho T2609) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10324 |
| Product name: | PRKDC (phospho T2609) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKDC. |
| Gene id: | 5591 |
| Gene name: | PRKDC |
| Gene alias: | DNA-PKcs|DNAPK|DNPK1|HYRC|HYRC1|XRCC7|p350 |
| Gene description: | protein kinase, DNA-activated, catalytic polypeptide |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding T2609 of human PRKDC. |
| Protein accession: | P78527;NP_008835 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:300000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal. |
| Reactivity: | Chicken,Chimpanzee,Dog,Horse,Human,Rat |
| Application image: |  |
| Application image note: | Western blot using PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324) shows detection of a 460 KDa band corresponding to human PRKDC in various preparations. Lane 1 : Fus1 untreated, Lane 2 : Fus1 IR (20Gy, 4h), Lane 3 : Fus1 PRKDC inhibitor + IR, Lane 4 : MO59J (DNAPK-) untreated, Lane 5 : MO59J IR, Lane 6 : Fus1 untreated, Lane 7 : Fus1 IR (20Gy, 4h), Lane 8 : Fus1 DNAPK inhibitor + IR, Lane 9 : MO59J untreated, Lane 10 : MO59J IR. Lanes 1~5 are nuclear extract whereas lanes 6~10 are whole cell lysates. MO59J is a cell line that lacks PRKDC. FUS1 is the matched cell line complemented with a chromosomal fragment containing the PRKDC gene. Approximately 20 $micro;g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1 : 1,000 dilution of PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324). Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection using a 1 min exposure time. |
| Applications: | ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | ATM and the catalytic subunit of DNA-dependent protein kinase activate NF-kappaB through a common MEK/extracellular signal-regulated kinase/p90(rsk) signaling pathway in response to distinct forms of DNA damage.Panta GR, Kaur S, Cavin LG, Cortes ML, Mercurio F, Lothstein L, Sweatman TW, Israel M, Arsura M. Mol Cell Biol. 2004 Mar;24(5):1823-35. |