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Data sheet of SUMO3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,IF,WB-Re |
More info about SUMO3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10320 |
| Product name: | SUMO3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against a recombinant SUMO3. |
| Gene id: | 6612 |
| Gene name: | SUMO3 |
| Gene alias: | SMT3A|SMT3H1|SUMO-3 |
| Gene description: | SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae) |
| Immunogen: | Recombinant protein corresponding to human SUMO3. |
| Protein accession: | NP_008867 |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot analysis is shown using SUMO3 polyclonal antibody (Cat # PAB10320) to detect GFP-SUMO fusion proteins (arrowheads). Panel A. Increasing concentrations of human GFP-SUMO3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1 : 1000 dilution of anti-SUMO3 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1 : 1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection. Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 ug of various GFP-SUMO constructs followed by transfer onto nitrocellulose. Lanes : 1, MW marker. 2, GFP-human SUMO3. 3, GFP-human SUMO1. 4, GFP-yeast SUMO. 5, GFP-Arabidopsis thaliana SUMO1. 6, GFP- Arabidopsis thaliana SUMO2. 7, GFP-tomato SUMO. After blocking for 1 hour with 5% non-fat dry milk in TTBS,the blot was probed overnight at 4°C with SUMO3 polyclonal antibody diluted and detected as above. Only the human GFP-SUMO3 band was visualized by chemiluminescence, and no crossreactivity with other SUMO family members was observed. |
| Applications: | ELISA,IF,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | SUMO, ubiquitin's mysterious cousin.Muller S, Hoege C, Pyrowolakis G, Jentsch S. Nat Rev Mol Cell Biol. 2001 Mar;2(3):202-10. |